Down’s syndrome cells ‘fixed’ in first step towards chromosome therapy
In an elegant series of experiments, US researchers took cells from people with DS and silenced the extra chromosome that causes the condition. Lawrence’s team used “genome editing”, a procedure that allows DNA to be cut and pasted, to drop a gene called XIST into the extra chromosome in cells taken from people with Down’s syndrome.
Previous studies found that the XIST gene is crucial for normal human development. The XIST gene sits on the X chromosome, but is only active in women. When it switches on, it silences the second X chromosome.
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Best Genomic Reagents at Best Prices by the Best Supplier!
*DNA/RNA Extraction & Purification
K-3011 DNA PrepMate™ II(150 extractions) – 257 EUR
DNA PrepMate™ -II uses a binding buffer and sillica matrix system being optimal condition to separate and purify DNA effectively.
Features and Benefits
Experiments Time: 15 min.(the case of PCR product purification), 40 min.(the case of plasmid extraction)
Sample source: Cultured bacterial cells, PCR products, Agarose gel fragments, Enzyme reaction mixtures, etc.
Application: PCR, Restriction enzyme reaction, Labeling, Cloning, Sequencing
K-3111 AccuPrep Nano_plus Plasmid Extraction Kit (200 extractions) – 235 EUR
Features and Benefits:
Principle is based on modified alkaline lysis protocol combined with a novel nano particle technology
Suitable for high-copy and low copy number plasmid DNA.
Highly purified and high purity plasmid DNA can be extracted from E.coli cells within 10 min.
Endonuclease A denaturation buffer for the endA+ strains (Denaturation Buffer, Buffer D).
Silica based DNA binding column with high DNA binding efficiency.
KB-0111 Proteinase K Powder (2X25 mg) – 193 EUR
K-3032 AccuPrep Genomic DNA Extraction Kit (100 extractions) – 246 EUR
Features and Benefits:
Highly purified and high yield genomic DNA can be extracted from Whole blood, Animal tissue, Cultured cells.
Silica based DNA binding column with high binding efficiency.
Optimized Tissue lysis buffer for efficient lysis of the various tissue sample.
*Order K-3032 + KB-0111 for just 314 EUR!!! You save 100 EUR!!!
K-3034 AccuPrep PCR Purification Kit PCR products (200 purifications) – 238 EUR
Features and Benefits:
Completely purify the fragment DNA from various enzymatic reaction products within 5 min.
Highly purified and high yield fragment DNA can be purified from various enzymatic reaction products (restriction enzyme digestion, A-tailing, labeling…).
Double strand and single strand DNA can be purified with high recovery.
Usable range is 100bp to 10kb with 70%-90% recovery.
Silica based DNA binding column with high binding efficiency.
*DNA Visualization
BH40501 Atlas ClearSight DNA Stain 1 ml – 70 EUR
DESCRIPTION:
Atlas ClearSight DNA Stain is a new nucleic acid stain that can be used as a safer alternative to the traditional Ethidium bromide stain for detecting nucleic acids in agarose gels. It is as sensitive as Ethidium bromide and can be used exactly the same way in agarose gel electrophoresis (Figure 1).
Atlas ClearSight DNA Stain emits green fluorescence when bound to DNA or RNA It has two secondary fluorescence excitation peaks (~270 nm; ~290 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is centered at ~530 nm. Thus, Atlas ClearSight DNA Stain is compatible with a wide variety of gel reading instruments.
Atlas ClearSight DNA Stain can be used for precast agarose gels and when better sensitivity is needed – poststaining is recommended
G108-P SafeView Colour Pack – 95 EUR
SafeView products represent a new and safe class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic Ethidium Bromide. The pack includes white, green and red dyes.
*PCR premixes and dNTPs
BH40203 Atlas dNTP Set 100 mM 4×0.4 ml – 118 EUR
DESCRIPTION:
Separate vials of four dNTPs
Concentrated neutralized solutions
Atlas dNTP Set contains four separate vials (4×0.4 ml) of dATP, dCTP, dGTP and dTTP, at a concentration of 100 mM each, in highly purified (>99%) deionized water at pH 7.5. Atlas dNTP Set is free of RNase and DNase, and suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, real-time PCR, DNA sequencing, cDNA synthesis and nick translation.
It contains everything you need for your PCR + a small expedient to ensure you the best results! Read about it HERE
*Assay kits for Sample preparation and DNA Methylation
P-1003-1 FitAmp General Tissue Section DNA Isolation Kit (50 samples) – 233 EUR
P-1011-1 Methylamp Universal Methylated DNA Kit – 268 EUR
P-1035-96 MethylFlash Methylated DNA Quantification Kit (Fluorometric) (96 assays) -637 EUR
P-1034-96 MethylFlash Methylated DNA Quantification Kit (Colorimetric) (96 assays) -575 EUR
P-1001-2 Methylamp DNA Modification– 244 EUR
P-1039-96 MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric) (96 assays) 506 EUR
P-1040-96 MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (fluorometric) (96 assays) – 506 EUR
The product is a complete set of optimized buffers and reagents to quantify 5-mC in fresh or frozen urine samples.
*Chromatin and Transcription
Chromatin structures are regulated by various mechanisms including histone modification and chromatin remodeling, which involve the binding of transcription factors.
P-2014-48 EpiQuik Plant ChIP Kit – 526 EUR*
P-2002-2 EpiQuik Chromatin Immunoprecipitation ChIP Kit – 409 EUR
P-2004-96 EpiQuik General Protein_DNA Binding Assay Kit Colorimetric –390 EUR
P-2006-48 EpiQuik Methyl_Histone H3K9 ChIP Kit (48 reactions)– 547 EUR*
P-2010-48 EpiQuik Acetyl_Histone H3 ChIP Kit (48 reactions)– 547 EUR*
P-2001-100 ChromaFlash Chromatin Extraction Kit (100 extractions) – 176 EUR
This kit is complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues.
*Histone Methylation
is the modification of certain amino acids in a histone protein by the addition of methyl groups. Quantifying histone methylation is particularly useful for studying gene expression patterns.
P-3015-096 EpiQuik In Situ Histone H3K4 Methylation Assay Kit– 434 EUR
P-3016-096 EpiQuik In Situ Histone H3K9 Methylation Assay Kit (96 assays)– 434 EUR*
*Gene Expressing and Knockout
Targeted gene knockdown using small interfereing RNA (siRNA) or antisense oligonucleotides has been valuable technology in studying gene function.
P-5001-48 QuantiSir General Gene Knockdown Quantification Kit 48 assays – 311 EUR
*CometAssay
The single cell gel electrophoresis or CometAssay® is a state-of-the-art technique for quantitating DNA damage and repair from in vivo and in vitro samples of eukaryotic cells and some prokaryotic cells.
4250-050-ES CometAssay Electrophoresis System1995 EUR/1596 EUR
250-050-ESK CometAssay Electrophoresis Starter Kit (includes CometAssay Kit, Control Cells & ES) 2112 EUR/1690 EUR
4253-096-03 96-Well CometSlide 94 EUR/ 75 EUR
*CometAssay Reagent Kits
4250-050-K CometAssay Kit269 EUR/ 215 EUR
4250-050-01 CometAssay Lysis Solution 118 EUR/ 94.4 EUR
4250-050-02 CometAssay LMAgarose89 EUR/71.2 EUR
*CometAssay Control Cells
4256-010-CC CometAssay Control Cells171 EUR/ 142 EUR
*Silver Staining Kit
For convenient silver staining of CometAssay® or single cell gel electrophoresis samples.
4251-050-K CometAssay Silver Kit 357 EUR/ 286 EUR
4254-200-K CometAssay Silver Staining Kit206 EUR/ 165 EUR
